The Determination of Uric Acid in Blood Serum

The isotachopherogram shown in figure 26 is that of patient suffering from gout.

Figure 26. The Determination of Uric Acid in Blood in a Hyperuracemic Serum

The leading electrolyte anion was chlorine ^–and the counter ion epsilon-aminocaproic acid at a concentration of 0.01M. The leading electrolyte was buffered at a pH of 5.0 and contained 0.25% hydroxy-etyl cellulose as an additive. The terminating electrolyte was morpholino-ethane-sulphonic acid and the counter ion tris-hydroxyethylaminomethane at a concentration of 0.05M. The terminating electrolyte was buffered at a pH of 6.5. It is seen that the urate is unambiguously identified by both resistance measurement and by measuring the UV absorption of the band.