The Basic Theoretical Model of the Isotachophoretic Process

Consider two monovalent anions (A) and (B) having mobilities () and () respectively

and

_{Assume a counter monovalent ion (q) is present in both zones and has a mobility ().}

_{Now for the system to be electronically neutral.}

_and

where () and () are concentration of (A) and (q) in band 1 and () and () are the concentrations of (B) and (q) in band 2.

Now all the zones must have equal velocities. Thus, or

and

_{Also as I is constant throughout the system.}

Now from Ohms Law or

Where the symbols have the meaning previously ascribed to them.

Now,

Where (c) is ion concentration in equivalents, (F) is Faradays Constant and (v) is the ion velocity.

Hence and as    then

Returning to the two monovalent anions (A) and (B) having mobilities () and () respectively in contact with counter ions (q) where () and () are concentration of (A) and (q) in band 1 and () and () are the concentrations of (B) and (q) in band 2.

Hence , and as   then

And by similar argument for band 2,

and as   then

Consequently, as ^then

_{Now as already stated, for the system to be electrically neutral,}

_and

Thus, The concentration of all the zones will be determined by the concentration of the leading electrolyte and on the mobilities of the respective ions involved.